Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add filters








Language
Year range
1.
Mem. Inst. Oswaldo Cruz ; 103(3): 301-305, May 2008. ilus, tab
Article in English | LILACS | ID: lil-485225

ABSTRACT

Three Enterococcus faecalis and one Enterococcus faecium strains were characterized by plasmid profile, pulsed-field gel electrophoresis (PFGE) and determination of antimicrobial minimal inhibitory concentrations. VanA elements were characterized by Long PCR, overlapping PCR and DNA sequencing. Enterococcal strains showed resistance to vancomycin and harbored the vanA gene, and three these were teicoplanin susceptible while one showed intermediate resistance to teicoplanin. Two E. faecalis strains showed indistinguishable PFGE profile while the third was unrelated. E. faecalis strains showed a deletion in the right terminal region of the Tn1546-like element. The E. faecium strain showed an insertion element in the vanXY intergenic region. Mutations in VanA elements were not found. Rearrangements in the VanA element could be responsible for incongruities in genotype and phenotype in these strains.


Subject(s)
Humans , Anti-Bacterial Agents/pharmacology , Enterococcus faecalis , Enterococcus faecium , Teicoplanin/pharmacology , Vancomycin/pharmacology , Brazil , Bacterial Proteins/genetics , Carbon-Oxygen Ligases/genetics , DNA, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Enterococcus faecalis/drug effects , Enterococcus faecalis/genetics , Enterococcus faecalis/isolation & purification , Enterococcus faecium/drug effects , Enterococcus faecium/genetics , Enterococcus faecium/isolation & purification , Genotype , Microbial Sensitivity Tests , Phenotype , Polymerase Chain Reaction , Sequence Analysis, DNA , Vancomycin Resistance/genetics
SELECTION OF CITATIONS
SEARCH DETAIL